Directed evolution of proteins by using novel engineering of genetic codes
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Life/Health/Medical informatics,Life/Health/Medical informatics,Life/Health/Medical informatics,Life/Health/Medical informatics
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background
● Directed Evolution allows developments of therapeutic proteins and industrial enzymes.
● ; unmodified therapeutic proteins are unstable and have immunogenicity.
● Poly ethylene glycol (PEG) modification circumvents the above problems ; modification at a random site decreases protein activity.
● Although protein mutants without Lysine residue can be site-specifically modified, design of such mutants is difficult.
● ; directed evolution often shows incomplete increase in properties of such proteins.
summary
● Our directed evolution creates a protein which has only one modification site.
● The key for the above evolution is our “simplified genetic codes” which do not encode Lysine.
● Our ”moving average genetic code” keeps increasing of a desired property of proteins during directed evolution.
● The key for the above evolution is our “simplified genetic codes” which do not encode Lysine.
● Our ”moving average genetic code” keeps increasing of a desired property of proteins during directed evolution.
predominance
● Site-specific modification of proteins by using conventional reagents.
● Taking full advantage of increase of protein activity by directed evolution.
● Creation of protein mutants which can not be obtained by the other methods.
● Large scale production with conventional culture and purification procedure is available for any mutant proteins evolved with our engineered genetic code.
● Taking full advantage of increase of protein activity by directed evolution.
● Creation of protein mutants which can not be obtained by the other methods.
● Large scale production with conventional culture and purification procedure is available for any mutant proteins evolved with our engineered genetic code.
application/development
● Therapeutic proteins.
● Enzymes used in oxidized conditions (ex. washing detergent).
● Proteins used in industries.
● Enzymes used in oxidized conditions (ex. washing detergent).
● Proteins used in industries.
posted:
2018/09/27