The differences in physical activity for opponent changes remain unclear for all organized sports. This study aimed to investigate the relationship between game results and activity profiles of the national men’s lacrosse team during the 2018 Federation of International Lacrosse World Championship. The study population included all twenty-one lacrosse players (five attacks: ten midfielders, and six defenses) of one team that participated in the Championship. The results revealed that in seven international matches, the distance was significantly greater in the higher-ranked group (the higher-ranked four teams of this study opponent) than in the lower-ranked group (the lower-ranked three teams of this study opponent), as was also the case for the walk and sprint distances (p < 0.05 for both comparisons). The sprint and high-speed running distances in the preliminary group (the team that competed in the qualifier) were significantly shorter than those in the final round group (the team that competed in the knockout phase) (p < 0.01 for both comparisons). The midfield group’s top speed and sprint distance were significantly greater than those of the attack and defense groups (p < 0.001). Multiple comparison tests showed that distance and jog distance in the 4th quarter (4Q) were significantly greater than those in 1Q–3Q (p < 0.01). Run and sprint distances and medium-intensity decelerations were significantly greater than in the 2Q (p <0.001, p =0.02 and p=0.049, respectively). The 4Q had significantly greater low-intensity accelerations and decelerations than the 2Q and 3Q (p < 0.05 for both comparisons). The differences in the opponent’s physical data can help coaches adjust training and conditioning before a match.