表題番号:2020C-276 日付:2021/03/31
研究課題マクロファージの多点連続刺激の細胞内での情報処理機構の解明
研究者所属(当時) 資格 氏名
(代表者) 理工学術院 先進理工学部 教授 安田 賢二
研究成果概要
Zipper model explained the driving mechanism of the engulfment procedure and its specific identification of antigens during phagocytosis in macrophages. To confirm the ability and limitation of this Zipper model in the critical conditions, we observed the phagocytosis after reached to the maximum engulfment capacity and the phagocytosis of the clusters of the IgG-coated and non-coated polystyrene sphere mixtures. We tracked the progression of membrane engulfing the IgG-coated polystyrene beads and IgG-coated microneedles. For the cluster engulfment, we recorded the time course of the cluster of different size IgG-coated/non-coated polystyrene spheres to confirm their strict selection of target antigens from the mixture cluster. The results showed that the irregular membrane backtracking as the reverse phenomenon of engulfment was observed after macrophages reached the maximum engulfment capacity. When the selective engulfment of IgG-coated polystyrene spheres from the mixtures cluster was not always successful, and sometimes, non-coated polystyrene spheres were caught into the cell body accompany with engulfment of the IgG-coated polystyrene sphere part of the cluster. In contrast, no membrane backtracking was observed during the engulfment of clusters. These results indicate that the mechanism of engulfment should imply the function of regurgitation for their survival and its recovery.