表題番号：2019C-563
日付：2020/06/01
研究課題エピゲノム編集によるNodal遺伝子ヒストン修飾制御の意義の解明
| 研究者所属（当時） | 資格 | 氏名 | |
|---|---|---|---|
| （代表者） | 理工学術院 先進理工学部 | 講師 | 新井 大祐 |
- 研究成果概要
- A goal of this study is to understand the true functions of trimethylation on histone H3 lysine 27 (H3K27me3) by CRISPR/dCas9-mediated epigenome editing. Here, we developed a series of expression vectors for H3K27me3 editing. The main components of H3K27me3 methyltransferase PRC2, including EZH2, EED, or JARID2, were adopted as the effector domain of dCas9. These vectors also carry a Puromycin-resistant gene, so that the transfectants could be rapidly selected by Puromycin treatment. The promoter region of Nodal gene was targeted by the CRISPR/dCas9-PRC2 vectors in mouse embryonic stem cells. As a result, dCas9-PRC2 caused mild but relatively long transcriptional repression of Nodal gene. This was in contrast to the case of dCas9-KRAB, which led to strong but very short repression through H3K9me3 deposition. It may suggest a difference between a mode of transcriptional repression by H3K27me3 and H3K9me3.