表題番号：2018S-126
日付：2019/04/08
研究課題アメーバ運動中の細胞内分子局在形成機構の解明
| 研究者所属（当時） | 資格 | 氏名 | |
|---|---|---|---|
| （代表者） | 理工学術院 先進理工学部 | 助手 | 山崎 陽祐 |
- 研究成果概要
- A cell moves by repeating expansion of the front and contraction of the rear. This cell movement requires proper intracellular localizations of actin binding proteins (ABPs), particularly segregation of myosin II and cofilin. The segregation mechanism remains unclear, but we have been studying possible involvement of the autonomy of the actin/ABP system. Myosin and cofilin form segregated patches along individual actin filaments, due to mutually exclusive/cooperative actin binding of the ABPs (Ngo et al., 2016), but each patch is too small to explain the cells-sized segregation of the two proteins in vivo. Here, we showed that the ABPs formed cell-sized segregation on the actin network whose density was high enough for each myosin filament to bind multiple actin filaments simultaneously. We also showed that the cell-sized segregation was not formed when myosin and cofilin were added simultaneously, but it was formed when myosin were added before cofilin. These results suggest that, in addition to the mutually exclusive/cooperative binding of the ABPs, the following two elements of the self-regulatory mechanism of actin/ABP system are important for the segregation. First, crosslinking of actin filaments by myosin filaments. Second, the physical condition that the filament-filament distance is smaller than the size of a myosin filament, which makes the myosin crosslink effective.