表題番号：2014K-6210
日付：2015/04/14
研究課題亜鉛欠乏による成熟ラット網状赤血球細胞数と網状赤血球内ヘモグロビン濃度の動態
| 研究者所属（当時） | 資格 | 氏名 | |
|---|---|---|---|
| （代表者） | 人間科学学術院 人間科学部 | 教授 | 今泉 和彦 |
| （連携研究者） | 杏林大学医学部 | 助教 | 白土 健 |
- 研究成果概要
- Zinc is known to play an important role for immune-functions. Recently, we showed that zinc-deficiency in the weaned rats for 4 weeks increased the duration-dependently the number of total white blood cells, granulocytes (neutrophil, eosinophil and basophil) and monocytes in 2-4 wks, without changing the number of lymphocytes, T-lymphocytes, B-lymphocytes or natural killer (NK) cells. These results suggest that zinc-deficiency induced cell-specific changes in the distribution of granulocytes and lymphocytes in rats. However, precisely how zinc-deficiency affects the distribution of a variety of matured red cells, including the minor population of reticulocytes, and the cell specificity of the effects remain unclear. Therefore, we investigated the effects of zinc-deficiency on the circulating number of matured red cells and reticulocytes in adult rats. Male 10-week-old Sprague-Dawley rats were randomly divided into the zinc-deficient diet (ZDD:0.7mg Zn/kg diet) group and the control diet (CON:34.8mg Zn/kg diet) group and were fed for 10 weeks. The analyses of the number of matured red cells and reticulocytes, hemoglobin concentrations of matured red cells and reticulocytes, and hematocrit values during the experimental period were performed by a flow cytometry technique (Model SF-3000, Sysmex Co). Plasma zinc concentrations during the experimental period were 0.51-0.75 times significantly lower in the ZDD group than in the CON group. The numbers of matured red cells in both groups increased with the progress of the experimental period. However, the numbers of matured red cells in the ZDD group were gradually inhibited during the experimenal period, as compared with those in the CON group. The numbers of red blood cells of the ZDD group in 8-9 weeks were 0.89-0.90 times significantly lower than those of the CON group. On the contrary, the numbers of reticulocytes of the ZDD group in 2-6 weeks were 0.62-0.83 times significantlly lower than those of the CON group. The relative ratio of the number of reticulocytes to the number of total blood cells in 2-6 weeks were 0.62-0.86 times significantly lower in the ZDD group than in the CON group. However, no significant differences between both groups in the relative ratios were observed in 8-10 weeks. On the other hand, reticulocyte hemoglobin concentrations in 2-8 weeks were 1.03-1.07 times significantly higher in the ZDD group than in the CON group. These results indicate that zinc-deficiency in adult male rats induced reticulocytopenia in 2-6 weeks and then decreased number of matured red cells in 8-10 weeks. Further studies are needed to clarify the mechanism of zinc-deficiency-induced reticulocytopenia in adult male rats.