Method for monitoring the treatment effect of Myeloproliferative neoplasms

2019-1021-08
researcher's name
about researcher TSUNEDA, Satoshi Professor
affiliation
Faculty of Science and Engineering
research field
Biofunction/Bioprocess,Environmental engineering and reduction of environmental burden,Applied microbiology,Bacteriology (including mycology)
keyword

background

● JAK2 V617F point mutation is detected as one of the driver mutations of myeloproliferative neoplasms (MPNs)
● High JAK2 V617F allele burden associates with the poor prognosis of MPNs
● Clinical examination technique to estimate the therapeutic effects is required

summary

● Usage of the fluorescence-dye-labeled probe exhibiting fluorescent intensity as a  function of the amount of V617F allele
● JAK2 V617F allele burden can be quantified equivalent to the results by next- generation sequencing (NGS)
● Available for the monitoring of slight increase/decrease of V617F allele burden

predominance

● NGS-compatible determination precision with simple and cost-effective  workflow
● Precise quantification independent with the input gDNA amount
● Utilized to determine the therapeutic effect

application/development

● Clinical examination
● Prognosis predicting
● Drug screening

material

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same researcher's seeds

  • Diagnostic method for chronic myeloproliferative diseases – method for quantitatively analyzing the JAK2 genetic mutation –
  • New isolation and culturing method for microorganisms
  • Mutational analysis method for JAK2 genes
posted: 2019/10/21