A method of isolating reporter cells by a highly sensitive trap vector system
2018-1123-08
- researcher's name
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about researcher SEMBA, Kentaro Professor
- affiliation
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Faculty of Science and Engineering School of Advanced Science and Engineering
- keyword
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background
● For evaluation of some drug activities or gene functions, reporter cells are useful.
● Reporter cells can be directly isolated by a trap method using transposon vector systems.
● However, conventional vector systems had low efficiency of isolating reporter cells due to low expression of reporter genes.
● Reporter cells can be directly isolated by a trap method using transposon vector systems.
● However, conventional vector systems had low efficiency of isolating reporter cells due to low expression of reporter genes.
predominance
● To highly express the fluorescent reporter protein in isolation, GAL4-UAS system was employed.
summary
● Our developed trap vector has over 100-fold sensitivity compared to conventional vector systems.
● New vector system isolates reporter cells dramatically improve isolation of reporter cells.
● New vector system isolates reporter cells dramatically improve isolation of reporter cells.
application/development
● For evaluation of some drug activities or gene functions.
● Identification of genes responsive to any stimulations without using microarray.
● Identification of genes responsive to any stimulations without using microarray.
collaborative researchers
小林 雄太 (理工学術院 生命医科学学科) , 若林 佑太郎 (理工学術院 生命医科学学科)
same researcher's seeds
- High-throughput screening system with cultured cell lines
posted:
2018/11/30